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Literature summary for 2.3.1.158 extracted from

  • Feng, Y.; Zhang, Y.; Ding, W.; Wu, P.; Cao, X.; Xue, S.
    Expanding of phospholipid diacylglycerol acyltransferase (PDAT) from Saccharomyces cerevisiae as multifunctional biocatalyst with broad acyl donor/acceptor selectivity (2019), Appl. Biochem. Biotechnol., 188, 824-835 .
    View publication on PubMed
Search for more literature for 2.3.1.158

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 deglycosylated enzyme, SDS-PAGE Saccharomyces cerevisiae
95000
-
 glycosylated enzyme, SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P40345
-
-
Saccharomyces cerevisiae ATCC 204508 P40345
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein four glycosylation sites, the degree of glycosylation site N469 is the largest Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phospholipid + 1,2-diacyl-sn-glycerol the enzyme is capable of using broad acyl donors such as PA, PS, PG, monogalactosyldiacylglycerol, digalactosyldiacylglycerol, and acyl-CoA. The enzyme is more likely to use unsaturated acyl donors comparing 18:0/18:1 to 18:0/18:0 phospholipids. With regard to acyl acceptors, the enzyme prefers 1,2 to 1,3-diacylglycerol, while 12:0/12:0 1,3-diacylglycerol is identified as the optimal acyl acceptor, followed by 18:1/18:1 and 18:1/16:0 1,3-diacylglycerol Saccharomyces cerevisiae lysophospholipid + triacylglycerol
-
 ?
phospholipid + 1,2-diacyl-sn-glycerol the enzyme is capable of using broad acyl donors such as PA, PS, PG, monogalactosyldiacylglycerol, digalactosyldiacylglycerol, and acyl-CoA. The enzyme is more likely to use unsaturated acyl donors comparing 18:0/18:1 to 18:0/18:0 phospholipids. With regard to acyl acceptors, the enzyme prefers 1,2 to 1,3-diacylglycerol, while 12:0/12:0 1,3-diacylglycerol is identified as the optimal acyl acceptor, followed by 18:1/18:1 and 18:1/16:0 1,3-diacylglycerol Saccharomyces cerevisiae ATCC 204508 lysophospholipid + triacylglycerol
-
 ?

Synonyms

Synonyms Comment Organism
PDAT
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Saccharomyces cerevisiae

General Information

General Information Comment Organism
metabolism the enzyme is responsible for the last step of triacylglycerol synthesis in the acyl-CoA-independent pathway, catalyzing membrane lipid transformation Saccharomyces cerevisiae